杭州菁因康生物科技有限公司

2021年9月10-12日，菁因康生物科技将参加由中国蛋白药物质量联盟、广东省生物产业协会、广州医药行业协会、振威国际会展集团主办的第6届BTE广州国际生物技术大会暨展览会。诚挚邀请相关领域采购商及专业人士莅临展会现场，洽谈合作、共赢未来。

杭州菁因康生物科技有限公司开发的糖工程毕赤酵母表达系统具有与高等真核表达系统（HEK293细胞）相同的诸多优点，包括抗体空间折叠、人源糖基化修饰等；同时又具有生长快，易于基因操作等优势。

公司开发出IgG全长抗体酵母展示技术平台，展示的IgG抗体在分子结构、理化特性 和生物学功能方面与天然抗体相近。这项技术可以构建大容量IgG全长抗体展示库，通过FACS分选技术对抗体亲和力及表达水平同时进行筛选，选出的抗体可以直接进行动物实验和临床试验。在抗体成熟优化应用中，这项技术比噬菌体展示更具优势，噬菌体展示只能展示ScFv等抗体片段，通过结构转换形成的IgG抗体可能会失去活性。我们提供涵盖抗体人源化、亲和力成熟、表达生产在内的抗体基因工程改造一站式服务。

Hangzhou Genekine Biotech Co. Ltd has developed proprietary platforms of glycoengineered pichia pastoris and full-length IgG pichia display system. There are many advantages of these two platforms. Similar to mammalian cell (HEK293) expression system, glycoengineered pichia pastoris contains eukaryotic internal quality control apparatus for protein correct folding and human-like N-glycosylation. Full-length IgG is displayed on pichia surface as close to native form in molecular structure, biophysical property, and biological function.

FACS sorting for IgG pichia display system is based on antibody affinity and display level. The isolated antibody can be directly expressed for animal study and clinical trial. Selection of IgG in pichia display has no need of format conversion. However selection of ScFv in phage display requires format conversion into full-length IgG, which may cause the lose of activity. Unlike the mammalian cell display, our platform can operate in large library size, easy genetic manipulation, fast generation time, and low cost screening. We will provide one-stop shop service for antibody humanization, affinity maturation, and antibody production.