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Human ACPP

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Prostatic Acid Phosphatase/ACPP 研究背景
 

Prostatic acid phosphatase (PAP, or ACPP), also known as prostatic specific acid phosphatase (PSAP), is an enzyme produced by the prostate. As a non-specific phosphomonoesterase, Prostatic acid phosphatase synthetized and secreted into seminal plasma under androgenic control. The enzyme is a dimer of molecular weight around 100 kDa. Prostatic acid phosphatase is a clinically important protein for its relevance as a biomarker of prostate carcinoma. Furthermore, it has a potential role in fertilization. The major action of PAP is to dephosphorylate macromolecules with the help of catalytic residues (His(12) and Asp(258)) that are located in the cleft between two domains. Cellular prostatic acid phosphatase (cPAcP), an authentic tyrosine phosphatase, is proposed to function as a negative growth regulator of prostate cancer (PCa) cells in part through its dephosphorylation of ErbB-2. cPAcP functions as a neutral protein tyrosine phosphatase (PTP) in prostate cancer cells and dephosphorylates HER-2/ErbB-2/Neu (HER-2: human epidermal growth factor receptor-2) at the phosphotyrosine (p-Tyr) residues. Injection of the secretory isoform of PAP has potent antinociceptive effects in mouse models of chronic pain. This enzyme exhibits ecto-5'-nucleotidase activity, is widely distributed, and implicated in the formation of chronic pain. Additionally, PAP could be a target molecule in specific immunotherapy for patients with nonprostate adenocarcinomas including colon and gastric cancers.

 
 
Human Prostatic Acid Phosphatase/ACPP 参考资料
 
  • Hassan MI, et al. (2010) Structural and functional analysis of human prostatic acid phosphatase. Expert Rev Anticancer Ther. 10(7): 1055-68.
  • Chuang TD, et al. (2010) Human prostatic acid phosphatase, an authentic tyrosine phosphatase, dephosphorylates ErbB-2 and regulates prostate cancer cell growth. J Biol Chem. 285(31): 23598-606.
  • Larsen RS, et al. (2009) A high throughput assay to identify small molecule modulators of prostatic acid phosphatase. Curr Chem Genomics. 3: 42-9.
  • Zimmermann H. (2009) Prostatic acid phosphatase, a neglected ectonucleotidase. Purinergic Signal. 5(3): 273-5.
  • Wang Y, et al. (2005) Prostatic acid phosphatase as a target molecule in specific immunotherapy for patients with nonprostate adenocarcinoma. J Immunother. 28(6): 535-41.
  • Veeramani S, et al. (2005) Cellular prostatic acid phosphatase: a protein tyrosine phosphatase involved in androgen-independent proliferation of prostate cancer. Endocr Relat Cancer. 12(4): 805-22.
  • Ostrowski WS, et al. (1994) Human prostatic acid phosphatase: selected properties and practical applications. Clin Chim Acta. 226(2): 121-9.
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    Human ACPP 蛋白产品信息
     
    分子别称: ACP-3, ACP3, PAP
    蛋白构建: A DNA sequence encoding the human ACPP (NP_001127666.1) (Met 1-Lys 382) was fused with a polyhistidine tag at the C-terminus.
    分子种属: Human
    表达宿主: Human Cells
    运输说明: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
    Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
     

     
    Human ACPP 蛋白质控检测
     
    纯度: > 97 % as determined by SDS-PAGE
    生物活性: Measured by its ability to cleave a substrate, pNitrophenyl phosphate (pNPP). The specific activity is >100,000 pmol/min/μg.
    内毒素: < 1.0 EU per μg of the protein as determined by the LAL method
    稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
    预测N端: Lys 33
    分子量: The recombinant human ACPP consists of 361 amino acids and has a predicted molecular mass of 42 kDa. The apparent molecular mass of rh ACPP is approximately 45-55 kDa in SDS-PAGE under reducing conditions.
    缓冲液: Lyophilized from sterile PBS, pH 7.4
    1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
    2. Please contact us for any concerns or special requirements.
     

     

     

     

     
    Human ACPP 蛋白使用指导
     
    储存方法: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
    复溶条件: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
     

     

     

     

     
    Human ACPP 蛋白SDS-PAGE
     

     

    Human Prostatic Acid Phosphatase / ACPP Protein (His Tag) SDS-PAGE

     

     

     

     
    Other ACPP 蛋白 Products
     

     

     

     

     

     

    10959-H08H

     

     

     

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